• Constantino Carlos Reyes-Aldasoro
• Research
• • Volumetric Texture Analysis
  • Measurement of Permeability
  • Tracking Red Blood Cells
  • Immune Cell Tracking and Analysis
  • Segmentation of Immunostained Microvessels
  • Measurement of Cellular Migration
  • Shading Correction
  • Tracing of Microvessels
  • CAIMAN: CAncer IMage ANalysis
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Measuring cellular migration with image processing

An image-processing algorithm for the analysis of migration of vascular endothelial cells in culture is presented. The algorithm correctly detected the cellular regions on either side of an artificial wound made by dragging a sterile pipette tip across the monolayer of cells (scratch wound assay). Frequency filtering and mathematical morphology were used to approximate the boundaries of cellular regions. This allowed the measurement of the distance between the regions, and therefore the migration rates, regardless of the orientation of the wound and even in cases where the cells were sparse and not tightly packed.

This algorithm was published in Electronics Letters.